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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
174 N V 0.30 7.0 25
174 N L 0.56 7.0 25
174 N I 0.50 7.0 25
174 N M 0.44 7.0 25
174 N F -0.17 7.0 25
174 N W -0.65 7.0 25
174 N Y -0.53 7.0 25
174 N G -3.80 7.0 25
174 N A -2.31 7.0 25
174 N P -1.19 7.0 25
174 N S -1.35 7.0 25
174 N T -1.35 7.0 25
174 N C -0.92 7.0 25
174 N H -1.88 7.0 25
174 N R -0.93 7.0 25
174 N K -1.65 7.0 25
174 N Q -1.75 7.0 25
174 N E -0.79 7.0 25
174 N D -1.63 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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