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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
175 G V -0.78 7.0 25
175 G L -0.41 7.0 25
175 G I -0.72 7.0 25
175 G M -0.48 7.0 25
175 G F -0.76 7.0 25
175 G W -0.92 7.0 25
175 G Y -1.17 7.0 25
175 G A -1.65 7.0 25
175 G P -1.14 7.0 25
175 G S -1.37 7.0 25
175 G T -1.65 7.0 25
175 G C -0.71 7.0 25
175 G H -1.18 7.0 25
175 G R -0.90 7.0 25
175 G K -1.36 7.0 25
175 G Q -0.99 7.0 25
175 G E -0.20 7.0 25
175 G N -0.90 7.0 25
175 G D -1.24 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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