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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
176 T V 0.01 7.0 25
176 T L -0.36 7.0 25
176 T I -0.87 7.0 25
176 T M 0.33 7.0 25
176 T F 0.39 7.0 25
176 T W 0.17 7.0 25
176 T Y 0.89 7.0 25
176 T G -1.58 7.0 25
176 T A -0.99 7.0 25
176 T P -1.28 7.0 25
176 T S -0.24 7.0 25
176 T C -0.01 7.0 25
176 T H -0.37 7.0 25
176 T R 0.22 7.0 25
176 T K -0.39 7.0 25
176 T Q -1.19 7.0 25
176 T E 0.25 7.0 25
176 T N -0.07 7.0 25
176 T D 0.41 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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