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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
177 V L -1.00 7.0 25
177 V I -1.47 7.0 25
177 V M -1.62 7.0 25
177 V F -1.99 7.0 25
177 V W -1.55 7.0 25
177 V Y -2.07 7.0 25
177 V G -3.71 7.0 25
177 V A -1.83 7.0 25
177 V P -2.68 7.0 25
177 V S -2.54 7.0 25
177 V T -1.64 7.0 25
177 V C -1.05 7.0 25
177 V H -1.39 7.0 25
177 V R -2.29 7.0 25
177 V K -2.14 7.0 25
177 V Q -2.27 7.0 25
177 V E -1.30 7.0 25
177 V N -2.53 7.0 25
177 V D -1.80 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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