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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
6 F V -2.38 7.0 25
6 F L -2.31 7.0 25
6 F I -0.99 7.0 25
6 F M -1.44 7.0 25
6 F W -0.93 7.0 25
6 F Y -1.51 7.0 25
6 F G -4.33 7.0 25
6 F A -2.63 7.0 25
6 F P -2.33 7.0 25
6 F S -2.45 7.0 25
6 F T -1.91 7.0 25
6 F C -2.41 7.0 25
6 F H -3.16 7.0 25
6 F R -2.17 7.0 25
6 F K -2.23 7.0 25
6 F Q -2.28 7.0 25
6 F E -1.72 7.0 25
6 F N -2.71 7.0 25
6 F D -2.39 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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