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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
143 T V 0.35 7.0 25
143 T L -0.38 7.0 25
143 T I 0.04 7.0 25
143 T M 0.24 7.0 25
143 T F 0.07 7.0 25
143 T W -0.35 7.0 25
143 T Y -0.16 7.0 25
143 T G -1.62 7.0 25
143 T A -0.60 7.0 25
143 T P -0.83 7.0 25
143 T S -0.18 7.0 25
143 T C -0.38 7.0 25
143 T H -1.85 7.0 25
143 T R -0.15 7.0 25
143 T K -0.52 7.0 25
143 T Q -1.07 7.0 25
143 T E -0.06 7.0 25
143 T N -0.72 7.0 25
143 T D 0.34 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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