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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
178 H V -1.02 7.0 25
178 H L -0.40 7.0 25
178 H I -1.05 7.0 25
178 H M -0.82 7.0 25
178 H F -0.63 7.0 25
178 H W -0.72 7.0 25
178 H Y -0.68 7.0 25
178 H G -1.38 7.0 25
178 H A -1.36 7.0 25
178 H P -1.45 7.0 25
178 H S -1.62 7.0 25
178 H T -1.16 7.0 25
178 H C -0.43 7.0 25
178 H R -1.49 7.0 25
178 H K -1.31 7.0 25
178 H Q -2.07 7.0 25
178 H E -0.52 7.0 25
178 H N -2.63 7.0 25
178 H D -1.64 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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