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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
2 I V -0.36 7.0 25
2 I L 0.75 7.0 25
2 I M -0.13 7.0 25
2 I F -0.36 7.0 25
2 I W -1.71 7.0 25
2 I Y -2.09 7.0 25
2 I G -2.65 7.0 25
2 I A -0.94 7.0 25
2 I P -2.35 7.0 25
2 I S -1.57 7.0 25
2 I T -1.71 7.0 25
2 I C -0.76 7.0 25
2 I H -1.18 7.0 25
2 I R -0.23 7.0 25
2 I K -1.17 7.0 25
2 I Q -1.33 7.0 25
2 I E -1.27 7.0 25
2 I N -0.17 7.0 25
2 I D -1.71 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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