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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
179 A V -0.88 7.0 25
179 A L -0.73 7.0 25
179 A I -0.76 7.0 25
179 A M -0.36 7.0 25
179 A F -0.18 7.0 25
179 A W -0.73 7.0 25
179 A Y -0.65 7.0 25
179 A G -1.07 7.0 25
179 A P -1.52 7.0 25
179 A S -1.40 7.0 25
179 A T -0.82 7.0 25
179 A C -1.09 7.0 25
179 A H -0.66 7.0 25
179 A R -0.50 7.0 25
179 A K -0.55 7.0 25
179 A Q -1.31 7.0 25
179 A E -0.76 7.0 25
179 A N -0.70 7.0 25
179 A D -0.90 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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