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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
180 G V -1.21 7.0 25
180 G L -0.93 7.0 25
180 G I -1.00 7.0 25
180 G M -0.85 7.0 25
180 G F -0.80 7.0 25
180 G W -0.97 7.0 25
180 G Y -1.38 7.0 25
180 G A -1.47 7.0 25
180 G P -1.32 7.0 25
180 G S -1.86 7.0 25
180 G T -1.58 7.0 25
180 G C -1.15 7.0 25
180 G H -1.17 7.0 25
180 G R -1.10 7.0 25
180 G K -1.24 7.0 25
180 G Q -1.26 7.0 25
180 G E -0.16 7.0 25
180 G N -1.18 7.0 25
180 G D -1.29 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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