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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
181 P V -1.64 7.0 25
181 P L -1.56 7.0 25
181 P I -1.62 7.0 25
181 P M -1.58 7.0 25
181 P F -1.08 7.0 25
181 P W -1.81 7.0 25
181 P Y -2.16 7.0 25
181 P G -2.05 7.0 25
181 P A -2.58 7.0 25
181 P S -2.72 7.0 25
181 P T -2.13 7.0 25
181 P C -1.73 7.0 25
181 P H -1.47 7.0 25
181 P R -1.58 7.0 25
181 P K -1.71 7.0 25
181 P Q -2.51 7.0 25
181 P E -1.22 7.0 25
181 P N -2.40 7.0 25
181 P D -1.91 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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