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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
5 E V 0.81 5.0 60
5 E L 1.00 5.0 60
5 E I 1.79 5.0 60
5 E M 0.55 5.0 60
5 E F 1.07 5.0 60
5 E W 0.49 5.0 60
5 E Y 0.22 5.0 60
5 E G -1.02 5.0 60
5 E A -0.18 5.0 60
5 E P 0.24 5.0 60
5 E S -0.23 5.0 60
5 E T 0.25 5.0 60
5 E C 0.29 5.0 60
5 E H -0.94 5.0 60
5 E R 0.48 5.0 60
5 E K -0.80 5.0 60
5 E Q -0.30 5.0 60
5 E N -0.73 5.0 60
5 E D -0.04 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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