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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
21 A V 0.33 7.0 25
21 A L 0.59 7.0 25
21 A I 0.61 7.0 25
21 A M 0.94 7.0 25
21 A F 0.01 7.0 25
21 A W -0.64 7.0 25
21 A Y -0.74 7.0 25
21 A G -0.82 7.0 25
21 A P -0.71 7.0 25
21 A S 0.21 7.0 25
21 A T -0.64 7.0 25
21 A C 0.43 7.0 25
21 A H -1.05 7.0 25
21 A R -0.02 7.0 25
21 A K -0.74 7.0 25
21 A Q -0.77 7.0 25
21 A E -0.68 7.0 25
21 A N -0.67 7.0 25
21 A D -0.76 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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