I-Mutant2.0

Last Update 27/12/06



        
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**                                                                       **
**                             I-Mutant v2.0                             **
**         Predictor of Protein Stability Changes upon Mutations         **
**                                                                       **
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      SEQ File: fileseq.txt

      Position   WT  NEW     DDG    pH    T
           211    G    V   -1.64   7.0   25
           211    G    L   -1.24   7.0   25
           211    G    I   -1.16   7.0   25
           211    G    M   -1.02   7.0   25
           211    G    F   -0.69   7.0   25
           211    G    W   -1.01   7.0   25
           211    G    Y   -1.30   7.0   25
           211    G    A   -1.27   7.0   25
           211    G    P   -1.90   7.0   25
           211    G    S   -1.31   7.0   25
           211    G    T   -1.41   7.0   25
           211    G    C   -1.40   7.0   25
           211    G    H   -1.56   7.0   25
           211    G    R   -1.32   7.0   25
           211    G    K   -1.54   7.0   25
           211    G    Q   -1.45   7.0   25
           211    G    E    0.14   7.0   25
           211    G    N   -1.22   7.0   25
           211    G    D   -1.14   7.0   25


      WT:  Aminoacid in Wild-Type Protein
      NEW: New Aminoacid after Mutation
      DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
             DDG<0: Decrease Stability
             DDG>0: Increase Stability
      T:   Temperature in Celsius degrees
      pH:  -log[H+]



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*                                                                         *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting  *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310.                                         *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
*                                                                         *
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