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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
7 I V -1.43 5.0 60
7 I L -1.11 5.0 60
7 I M -2.36 5.0 60
7 I F -2.55 5.0 60
7 I W -2.91 5.0 60
7 I Y -3.77 5.0 60
7 I G -4.98 5.0 60
7 I A -3.80 5.0 60
7 I P -3.05 5.0 60
7 I S -3.65 5.0 60
7 I T -4.03 5.0 60
7 I C -3.40 5.0 60
7 I H -3.70 5.0 60
7 I R -2.62 5.0 60
7 I K -3.39 5.0 60
7 I Q -3.12 5.0 60
7 I E -2.55 5.0 60
7 I N -2.26 5.0 60
7 I D -3.46 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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