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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
236 R V -0.52 7.0 25
236 R L -0.42 7.0 25
236 R I -0.20 7.0 25
236 R M -1.18 7.0 25
236 R F -0.29 7.0 25
236 R W -0.51 7.0 25
236 R Y -0.49 7.0 25
236 R G -1.21 7.0 25
236 R A -1.08 7.0 25
236 R P -1.18 7.0 25
236 R S -2.88 7.0 25
236 R T -1.90 7.0 25
236 R C -0.65 7.0 25
236 R H -1.77 7.0 25
236 R K -1.58 7.0 25
236 R Q -1.07 7.0 25
236 R E -0.80 7.0 25
236 R N -1.46 7.0 25
236 R D -1.66 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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