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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
9 V L -0.68 5.0 60
9 V I -0.02 5.0 60
9 V M -2.07 5.0 60
9 V F -2.67 5.0 60
9 V W -1.43 5.0 60
9 V Y -2.06 5.0 60
9 V G -3.73 5.0 60
9 V A -2.10 5.0 60
9 V P -2.57 5.0 60
9 V S -2.58 5.0 60
9 V T -2.49 5.0 60
9 V C -0.71 5.0 60
9 V H -2.40 5.0 60
9 V R -1.97 5.0 60
9 V K -2.48 5.0 60
9 V Q -2.21 5.0 60
9 V E -1.53 5.0 60
9 V N -3.39 5.0 60
9 V D -1.75 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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