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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
10 Q V 0.64 5.0 60
10 Q L 0.44 5.0 60
10 Q I 1.20 5.0 60
10 Q M -0.76 5.0 60
10 Q F 0.19 5.0 60
10 Q W 0.35 5.0 60
10 Q Y 0.35 5.0 60
10 Q G -0.72 5.0 60
10 Q A 0.17 5.0 60
10 Q P -0.63 5.0 60
10 Q S -0.73 5.0 60
10 Q T -0.39 5.0 60
10 Q C 0.94 5.0 60
10 Q H -0.95 5.0 60
10 Q R -0.46 5.0 60
10 Q K -0.18 5.0 60
10 Q E 0.61 5.0 60
10 Q N -2.24 5.0 60
10 Q D -0.17 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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