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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
116 A V -0.15 7.0 25
116 A L 0.41 7.0 25
116 A I -0.22 7.0 25
116 A M -0.32 7.0 25
116 A F -0.71 7.0 25
116 A W -1.03 7.0 25
116 A Y -1.03 7.0 25
116 A G -1.16 7.0 25
116 A P -1.65 7.0 25
116 A S -0.71 7.0 25
116 A T -1.31 7.0 25
116 A C -0.05 7.0 25
116 A H -1.50 7.0 25
116 A R -0.32 7.0 25
116 A K -1.34 7.0 25
116 A Q -0.43 7.0 25
116 A E -0.87 7.0 25
116 A N -0.59 7.0 25
116 A D -1.93 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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