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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
238 W V -0.83 7.0 25
238 W L -0.62 7.0 25
238 W I -1.15 7.0 25
238 W M -1.69 7.0 25
238 W F -1.47 7.0 25
238 W Y -1.22 7.0 25
238 W G -3.28 7.0 25
238 W A -2.35 7.0 25
238 W P -1.81 7.0 25
238 W S -2.70 7.0 25
238 W T -2.74 7.0 25
238 W C -1.21 7.0 25
238 W H -2.50 7.0 25
238 W R -1.25 7.0 25
238 W K -2.38 7.0 25
238 W Q -1.99 7.0 25
238 W E -1.68 7.0 25
238 W N -2.12 7.0 25
238 W D -2.22 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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