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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
15 R V 0.21 7.0 25
15 R L 0.27 7.0 25
15 R I 0.25 7.0 25
15 R M -0.02 7.0 25
15 R F 0.04 7.0 25
15 R W -0.71 7.0 25
15 R Y -0.39 7.0 25
15 R G -0.45 7.0 25
15 R A -0.06 7.0 25
15 R P -0.67 7.0 25
15 R S -1.72 7.0 25
15 R T -0.86 7.0 25
15 R C 0.20 7.0 25
15 R H -0.53 7.0 25
15 R K -1.08 7.0 25
15 R Q -0.49 7.0 25
15 R E -0.58 7.0 25
15 R N -0.89 7.0 25
15 R D -1.38 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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