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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
231 D V 0.31 5.0 60
231 D L -0.07 5.0 60
231 D I 0.06 5.0 60
231 D M 0.22 5.0 60
231 D F -0.01 5.0 60
231 D W -0.27 5.0 60
231 D Y -0.13 5.0 60
231 D G 0.54 5.0 60
231 D A 0.43 5.0 60
231 D P -0.88 5.0 60
231 D S 0.17 5.0 60
231 D T -0.32 5.0 60
231 D C 0.77 5.0 60
231 D H 0.02 5.0 60
231 D R 0.71 5.0 60
231 D K -0.14 5.0 60
231 D Q 0.54 5.0 60
231 D E 0.22 5.0 60
231 D N -0.49 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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