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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
3 A V -0.05 7.0 25
3 A L 0.49 7.0 25
3 A I 0.38 7.0 25
3 A M -0.31 7.0 25
3 A F -0.06 7.0 25
3 A W -1.05 7.0 25
3 A Y -1.28 7.0 25
3 A G -2.07 7.0 25
3 A P -1.52 7.0 25
3 A S -0.50 7.0 25
3 A T -1.05 7.0 25
3 A C -0.03 7.0 25
3 A H -1.59 7.0 25
3 A R -0.04 7.0 25
3 A K -1.45 7.0 25
3 A Q -0.94 7.0 25
3 A E -1.22 7.0 25
3 A N -0.97 7.0 25
3 A D -1.42 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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