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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
12 H V 0.95 5.0 60
12 H L 1.57 5.0 60
12 H I 1.47 5.0 60
12 H M 0.44 5.0 60
12 H F 0.80 5.0 60
12 H W 1.00 5.0 60
12 H Y 0.96 5.0 60
12 H G 0.15 5.0 60
12 H A 0.56 5.0 60
12 H P 0.37 5.0 60
12 H S 0.18 5.0 60
12 H T 0.18 5.0 60
12 H C 1.53 5.0 60
12 H R -0.03 5.0 60
12 H K 0.16 5.0 60
12 H Q -0.31 5.0 60
12 H E 0.89 5.0 60
12 H N -1.64 5.0 60
12 H D -0.12 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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