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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
13 L V -0.81 5.0 60
13 L I -0.20 5.0 60
13 L M 0.11 5.0 60
13 L F 0.19 5.0 60
13 L W 0.41 5.0 60
13 L Y -0.23 5.0 60
13 L G -3.53 5.0 60
13 L A -2.34 5.0 60
13 L P -0.72 5.0 60
13 L S -2.38 5.0 60
13 L T -2.62 5.0 60
13 L C -2.14 5.0 60
13 L H -1.58 5.0 60
13 L R -1.83 5.0 60
13 L K -2.17 5.0 60
13 L Q -1.52 5.0 60
13 L E -0.75 5.0 60
13 L N -2.46 5.0 60
13 L D -3.14 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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