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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
240 L V -0.49 7.0 25
240 L I -0.34 7.0 25
240 L M -0.72 7.0 25
240 L F 0.28 7.0 25
240 L W 0.20 7.0 25
240 L Y -0.13 7.0 25
240 L G -3.64 7.0 25
240 L A -2.61 7.0 25
240 L P -0.36 7.0 25
240 L S -2.77 7.0 25
240 L T -4.01 7.0 25
240 L C -2.17 7.0 25
240 L H -1.35 7.0 25
240 L R -0.45 7.0 25
240 L K -2.28 7.0 25
240 L Q -1.49 7.0 25
240 L E -1.18 7.0 25
240 L N -2.35 7.0 25
240 L D -3.44 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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