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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
241 P V -0.47 7.0 25
241 P L -0.50 7.0 25
241 P I -0.44 7.0 25
241 P M -1.22 7.0 25
241 P F -0.66 7.0 25
241 P W -1.18 7.0 25
241 P Y -1.56 7.0 25
241 P G -1.72 7.0 25
241 P A -2.13 7.0 25
241 P S -2.12 7.0 25
241 P T -2.64 7.0 25
241 P C -1.00 7.0 25
241 P H -1.97 7.0 25
241 P R -0.49 7.0 25
241 P K -1.47 7.0 25
241 P Q -1.54 7.0 25
241 P E -1.07 7.0 25
241 P N -1.89 7.0 25
241 P D -1.93 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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