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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
242 N V 1.18 7.0 25
242 N L 0.40 7.0 25
242 N I 1.18 7.0 25
242 N M -0.03 7.0 25
242 N F -0.01 7.0 25
242 N W -0.32 7.0 25
242 N Y -0.36 7.0 25
242 N G -1.75 7.0 25
242 N A -1.30 7.0 25
242 N P -0.37 7.0 25
242 N S -1.35 7.0 25
242 N T -1.87 7.0 25
242 N C -0.56 7.0 25
242 N H -2.31 7.0 25
242 N R -0.17 7.0 25
242 N K -1.09 7.0 25
242 N Q -1.04 7.0 25
242 N E -0.39 7.0 25
242 N D -1.81 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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