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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
243 Y V 0.87 7.0 25
243 Y L 0.32 7.0 25
243 Y I 0.33 7.0 25
243 Y M -0.58 7.0 25
243 Y F -0.02 7.0 25
243 Y W 0.09 7.0 25
243 Y G -2.62 7.0 25
243 Y A -1.22 7.0 25
243 Y P -0.35 7.0 25
243 Y S -1.28 7.0 25
243 Y T -1.08 7.0 25
243 Y C 0.96 7.0 25
243 Y H -0.87 7.0 25
243 Y R 0.18 7.0 25
243 Y K -0.20 7.0 25
243 Y Q -0.09 7.0 25
243 Y E -0.05 7.0 25
243 Y N -1.00 7.0 25
243 Y D -0.69 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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