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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
15 S V 0.55 5.0 60
15 S L -0.13 5.0 60
15 S I 0.75 5.0 60
15 S M 0.04 5.0 60
15 S F 0.45 5.0 60
15 S W 0.34 5.0 60
15 S Y -0.33 5.0 60
15 S G -1.30 5.0 60
15 S A -0.23 5.0 60
15 S P -0.92 5.0 60
15 S T 0.32 5.0 60
15 S C -1.02 5.0 60
15 S H -0.87 5.0 60
15 S R -0.82 5.0 60
15 S K 0.12 5.0 60
15 S Q -0.49 5.0 60
15 S E 0.15 5.0 60
15 S N -0.40 5.0 60
15 S D -0.07 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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