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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
256 V L -1.86 7.0 25
256 V I -0.53 7.0 25
256 V M -1.82 7.0 25
256 V F -2.51 7.0 25
256 V W -1.45 7.0 25
256 V Y -2.12 7.0 25
256 V G -4.24 7.0 25
256 V A -2.64 7.0 25
256 V P -2.90 7.0 25
256 V S -3.01 7.0 25
256 V T -2.32 7.0 25
256 V C -1.94 7.0 25
256 V H -2.35 7.0 25
256 V R -2.52 7.0 25
256 V K -2.44 7.0 25
256 V Q -2.04 7.0 25
256 V E -1.59 7.0 25
256 V N -2.82 7.0 25
256 V D -1.61 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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