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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
244 Q V 0.79 7.0 25
244 Q L -0.19 7.0 25
244 Q I 0.64 7.0 25
244 Q M -1.40 7.0 25
244 Q F -0.04 7.0 25
244 Q W -0.29 7.0 25
244 Q Y -0.06 7.0 25
244 Q G -0.91 7.0 25
244 Q A -0.67 7.0 25
244 Q P -0.51 7.0 25
244 Q S -1.60 7.0 25
244 Q T -1.78 7.0 25
244 Q C -0.01 7.0 25
244 Q H -1.18 7.0 25
244 Q R -0.68 7.0 25
244 Q K -0.35 7.0 25
244 Q E 0.16 7.0 25
244 Q N -2.13 7.0 25
244 Q D -0.76 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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