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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
22 G V -0.62 5.0 60
22 G L -0.51 5.0 60
22 G I -0.01 5.0 60
22 G M -0.84 5.0 60
22 G F -1.08 5.0 60
22 G W -1.06 5.0 60
22 G Y -1.15 5.0 60
22 G A -0.54 5.0 60
22 G P -0.81 5.0 60
22 G S -1.71 5.0 60
22 G T -1.75 5.0 60
22 G C -1.07 5.0 60
22 G H -1.82 5.0 60
22 G R -1.74 5.0 60
22 G K -1.25 5.0 60
22 G Q -0.86 5.0 60
22 G E -0.09 5.0 60
22 G N -1.58 5.0 60
22 G D -1.61 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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