***************************************************************************
** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
***************************************************************************
SEQ File: fileseq.txt
Position WT NEW DDG pH T
65 T V 0.30 7.0 25
65 T L -0.41 7.0 25
65 T I 0.13 7.0 25
65 T M -0.37 7.0 25
65 T F 0.32 7.0 25
65 T W 0.00 7.0 25
65 T Y 1.57 7.0 25
65 T G -1.31 7.0 25
65 T A -0.71 7.0 25
65 T P -0.71 7.0 25
65 T S -1.00 7.0 25
65 T C -0.96 7.0 25
65 T H -1.03 7.0 25
65 T R -0.02 7.0 25
65 T K -0.50 7.0 25
65 T Q -1.11 7.0 25
65 T E 0.03 7.0 25
65 T N -0.30 7.0 25
65 T D -0.31 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
***************************************************************************
* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
***************************************************************************