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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
16 D V 0.23 5.0 60
16 D L 0.20 5.0 60
16 D I 0.41 5.0 60
16 D M 0.18 5.0 60
16 D F 0.48 5.0 60
16 D W 0.34 5.0 60
16 D Y 0.45 5.0 60
16 D G -0.25 5.0 60
16 D A 0.53 5.0 60
16 D P -0.51 5.0 60
16 D S 0.40 5.0 60
16 D T 0.39 5.0 60
16 D C -0.10 5.0 60
16 D H 0.22 5.0 60
16 D R -0.06 5.0 60
16 D K 0.22 5.0 60
16 D Q 0.28 5.0 60
16 D E 0.70 5.0 60
16 D N -0.47 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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