***************************************************************************
** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
***************************************************************************
SEQ File: fileseq.txt
Position WT NEW DDG pH T
23 E V -0.17 5.0 60
23 E L 0.61 5.0 60
23 E I 0.84 5.0 60
23 E M -0.02 5.0 60
23 E F 0.48 5.0 60
23 E W -0.36 5.0 60
23 E Y -0.14 5.0 60
23 E G -1.06 5.0 60
23 E A -0.85 5.0 60
23 E P -0.44 5.0 60
23 E S -1.26 5.0 60
23 E T -0.04 5.0 60
23 E C -0.49 5.0 60
23 E H -0.64 5.0 60
23 E R -0.77 5.0 60
23 E K -0.72 5.0 60
23 E Q -0.87 5.0 60
23 E N -1.44 5.0 60
23 E D -0.76 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
***************************************************************************
* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
***************************************************************************