***************************************************************************
** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
***************************************************************************
SEQ File: fileseq.txt
Position WT NEW DDG pH T
258 K V 1.10 7.0 25
258 K L 0.63 7.0 25
258 K I 0.94 7.0 25
258 K M 0.36 7.0 25
258 K F 1.72 7.0 25
258 K W 0.50 7.0 25
258 K Y 0.80 7.0 25
258 K G -0.26 7.0 25
258 K A 0.24 7.0 25
258 K P 1.22 7.0 25
258 K S -0.46 7.0 25
258 K T 0.19 7.0 25
258 K C -0.23 7.0 25
258 K H 0.20 7.0 25
258 K R -0.42 7.0 25
258 K Q 0.24 7.0 25
258 K E 0.83 7.0 25
258 K N 0.25 7.0 25
258 K D 0.32 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
***************************************************************************
* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
***************************************************************************