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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
24 T V -0.40 5.0 60
24 T L -0.19 5.0 60
24 T I -0.32 5.0 60
24 T M 0.13 5.0 60
24 T F 0.39 5.0 60
24 T W 0.20 5.0 60
24 T Y 1.28 5.0 60
24 T G -1.09 5.0 60
24 T A -0.38 5.0 60
24 T P -1.39 5.0 60
24 T S -0.19 5.0 60
24 T C -0.08 5.0 60
24 T H -0.74 5.0 60
24 T R -0.15 5.0 60
24 T K -0.38 5.0 60
24 T Q -1.02 5.0 60
24 T E 0.26 5.0 60
24 T N -0.35 5.0 60
24 T D 0.11 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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