***************************************************************************
** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
***************************************************************************
SEQ File: fileseq.txt
Position WT NEW DDG pH T
106 C V -0.95 7.0 25
106 C L -0.90 7.0 25
106 C I -0.56 7.0 25
106 C M -0.84 7.0 25
106 C F -0.79 7.0 25
106 C W -0.75 7.0 25
106 C Y -0.02 7.0 25
106 C G -0.79 7.0 25
106 C A -0.57 7.0 25
106 C P -1.43 7.0 25
106 C S -0.37 7.0 25
106 C T -1.32 7.0 25
106 C H -0.22 7.0 25
106 C R -1.05 7.0 25
106 C K -1.19 7.0 25
106 C Q -0.88 7.0 25
106 C E -0.47 7.0 25
106 C N -1.05 7.0 25
106 C D -1.28 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
***************************************************************************
* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
***************************************************************************