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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
155 G V -2.26 7.0 25
155 G L -1.55 7.0 25
155 G I -1.56 7.0 25
155 G M -1.50 7.0 25
155 G F -1.82 7.0 25
155 G W -1.77 7.0 25
155 G Y -1.81 7.0 25
155 G A -2.15 7.0 25
155 G P -2.58 7.0 25
155 G S -1.96 7.0 25
155 G T -2.49 7.0 25
155 G C -1.55 7.0 25
155 G H -1.99 7.0 25
155 G R -1.59 7.0 25
155 G K -1.93 7.0 25
155 G Q -1.87 7.0 25
155 G E -1.15 7.0 25
155 G N -2.15 7.0 25
155 G D -2.41 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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