***************************************************************************
** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
***************************************************************************
SEQ File: fileseq.txt
Position WT NEW DDG pH T
1266 E V 1.30 7.0 25
1266 E L 0.57 7.0 25
1266 E I 0.77 7.0 25
1266 E M 0.35 7.0 25
1266 E F 1.33 7.0 25
1266 E W 0.01 7.0 25
1266 E Y 0.58 7.0 25
1266 E G -1.74 7.0 25
1266 E A -1.30 7.0 25
1266 E P -0.55 7.0 25
1266 E S -1.00 7.0 25
1266 E T -0.71 7.0 25
1266 E C -0.47 7.0 25
1266 E H -1.16 7.0 25
1266 E R 0.19 7.0 25
1266 E K -1.50 7.0 25
1266 E Q -0.70 7.0 25
1266 E N -0.83 7.0 25
1266 E D -0.14 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
***************************************************************************
* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
***************************************************************************