I-Mutant2.0

Last Update 27/12/06



        
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**                                                                       **
**                             I-Mutant v2.0                             **
**         Predictor of Protein Stability Changes upon Mutations         **
**                                                                       **
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      SEQ File: fileseq.txt

      Position   WT  NEW     DDG    pH    T
           168    C    V   -0.31   5.0   60
           168    C    L    0.31   5.0   60
           168    C    I    0.19   5.0   60
           168    C    M   -0.48   5.0   60
           168    C    F   -0.13   5.0   60
           168    C    W   -0.30   5.0   60
           168    C    Y    0.37   5.0   60
           168    C    G   -1.10   5.0   60
           168    C    A   -0.13   5.0   60
           168    C    P   -0.73   5.0   60
           168    C    S   -0.67   5.0   60
           168    C    T   -0.51   5.0   60
           168    C    H   -0.33   5.0   60
           168    C    R   -0.42   5.0   60
           168    C    K   -0.53   5.0   60
           168    C    Q   -0.34   5.0   60
           168    C    E   -0.21   5.0   60
           168    C    N   -0.52   5.0   60
           168    C    D   -1.23   5.0   60


      WT:  Aminoacid in Wild-Type Protein
      NEW: New Aminoacid after Mutation
      DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
             DDG<0: Decrease Stability
             DDG>0: Increase Stability
      T:   Temperature in Celsius degrees
      pH:  -log[H+]



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*                                                                         *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting  *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310.                                         *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
*                                                                         *
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