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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
168 C V -0.31 5.0 60
168 C L 0.31 5.0 60
168 C I 0.19 5.0 60
168 C M -0.48 5.0 60
168 C F -0.13 5.0 60
168 C W -0.30 5.0 60
168 C Y 0.37 5.0 60
168 C G -1.10 5.0 60
168 C A -0.13 5.0 60
168 C P -0.73 5.0 60
168 C S -0.67 5.0 60
168 C T -0.51 5.0 60
168 C H -0.33 5.0 60
168 C R -0.42 5.0 60
168 C K -0.53 5.0 60
168 C Q -0.34 5.0 60
168 C E -0.21 5.0 60
168 C N -0.52 5.0 60
168 C D -1.23 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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