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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
259 V L -2.01 7.0 25
259 V I -1.11 7.0 25
259 V M -3.14 7.0 25
259 V F -3.27 7.0 25
259 V W -2.36 7.0 25
259 V Y -2.89 7.0 25
259 V G -5.23 7.0 25
259 V A -3.33 7.0 25
259 V P -3.51 7.0 25
259 V S -4.02 7.0 25
259 V T -2.91 7.0 25
259 V C -2.76 7.0 25
259 V H -2.78 7.0 25
259 V R -3.55 7.0 25
259 V K -3.15 7.0 25
259 V Q -2.99 7.0 25
259 V E -2.12 7.0 25
259 V N -4.16 7.0 25
259 V D -2.53 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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