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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
322 K V 0.85 7.0 25
322 K L 1.02 7.0 25
322 K I 0.53 7.0 25
322 K M 0.30 7.0 25
322 K F 2.05 7.0 25
322 K W 0.33 7.0 25
322 K Y 1.23 7.0 25
322 K G -0.96 7.0 25
322 K A 0.18 7.0 25
322 K P 0.58 7.0 25
322 K S -0.50 7.0 25
322 K T -0.09 7.0 25
322 K C 0.54 7.0 25
322 K H -0.15 7.0 25
322 K R 0.27 7.0 25
322 K Q -0.18 7.0 25
322 K E -0.28 7.0 25
322 K N 0.49 7.0 25
322 K D -0.73 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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