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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
27 Y V 0.08 5.0 60
27 Y L 0.80 5.0 60
27 Y I -0.03 5.0 60
27 Y M -0.03 5.0 60
27 Y F -0.14 5.0 60
27 Y W 0.08 5.0 60
27 Y G -2.54 5.0 60
27 Y A -0.81 5.0 60
27 Y P -0.76 5.0 60
27 Y S -1.33 5.0 60
27 Y T 0.35 5.0 60
27 Y C 0.88 5.0 60
27 Y H 0.19 5.0 60
27 Y R -0.83 5.0 60
27 Y K 0.03 5.0 60
27 Y Q -0.26 5.0 60
27 Y E 0.21 5.0 60
27 Y N -0.75 5.0 60
27 Y D -0.09 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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