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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
103 M V -0.03 7.5 50
103 M L -0.60 7.5 50
103 M I 0.04 7.5 50
103 M F 0.85 7.5 50
103 M W 0.04 7.5 50
103 M Y 0.11 7.5 50
103 M G -0.99 7.5 50
103 M A -0.70 7.5 50
103 M P -0.45 7.5 50
103 M S -0.36 7.5 50
103 M T 0.52 7.5 50
103 M C -0.19 7.5 50
103 M H 0.24 7.5 50
103 M R -0.14 7.5 50
103 M K -0.69 7.5 50
103 M Q -0.67 7.5 50
103 M E 0.38 7.5 50
103 M N -0.98 7.5 50
103 M D 0.46 7.5 50
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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