I-Mutant2.0

Last Update 27/12/06



        
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**                                                                       **
**                             I-Mutant v2.0                             **
**         Predictor of Protein Stability Changes upon Mutations         **
**                                                                       **
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      SEQ File: fileseq.txt

      Position   WT  NEW     DDG    pH    T
           173    G    V   -0.72   5.0   60
           173    G    L   -0.48   5.0   60
           173    G    I   -0.43   5.0   60
           173    G    M   -0.50   5.0   60
           173    G    F   -0.78   5.0   60
           173    G    W   -0.84   5.0   60
           173    G    Y   -1.12   5.0   60
           173    G    A   -0.84   5.0   60
           173    G    P   -0.92   5.0   60
           173    G    S   -1.35   5.0   60
           173    G    T   -1.36   5.0   60
           173    G    C   -0.42   5.0   60
           173    G    H   -1.00   5.0   60
           173    G    R   -0.82   5.0   60
           173    G    K   -0.93   5.0   60
           173    G    Q   -0.86   5.0   60
           173    G    E   -0.10   5.0   60
           173    G    N   -0.74   5.0   60
           173    G    D   -1.53   5.0   60


      WT:  Aminoacid in Wild-Type Protein
      NEW: New Aminoacid after Mutation
      DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
             DDG<0: Decrease Stability
             DDG>0: Increase Stability
      T:   Temperature in Celsius degrees
      pH:  -log[H+]



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*                                                                         *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting  *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310.                                         *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
*                                                                         *
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