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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
173 G V -0.72 5.0 60
173 G L -0.48 5.0 60
173 G I -0.43 5.0 60
173 G M -0.50 5.0 60
173 G F -0.78 5.0 60
173 G W -0.84 5.0 60
173 G Y -1.12 5.0 60
173 G A -0.84 5.0 60
173 G P -0.92 5.0 60
173 G S -1.35 5.0 60
173 G T -1.36 5.0 60
173 G C -0.42 5.0 60
173 G H -1.00 5.0 60
173 G R -0.82 5.0 60
173 G K -0.93 5.0 60
173 G Q -0.86 5.0 60
173 G E -0.10 5.0 60
173 G N -0.74 5.0 60
173 G D -1.53 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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