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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
243 G V -0.50 7.0 25
243 G L -1.05 7.0 25
243 G I -0.34 7.0 25
243 G M -0.76 7.0 25
243 G F -0.76 7.0 25
243 G W -1.01 7.0 25
243 G Y -1.24 7.0 25
243 G A -0.84 7.0 25
243 G P -1.05 7.0 25
243 G S -1.00 7.0 25
243 G T -1.51 7.0 25
243 G C -1.28 7.0 25
243 G H -1.79 7.0 25
243 G R -1.14 7.0 25
243 G K -1.65 7.0 25
243 G Q -0.84 7.0 25
243 G E 0.13 7.0 25
243 G N -1.35 7.0 25
243 G D -1.31 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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