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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
1 M V -0.73 7.0 25
1 M L -1.71 7.0 25
1 M I -0.66 7.0 25
1 M F 0.04 7.0 25
1 M W -0.90 7.0 25
1 M Y -1.01 7.0 25
1 M G -1.49 7.0 25
1 M A -1.22 7.0 25
1 M P -1.32 7.0 25
1 M S -0.57 7.0 25
1 M T -0.52 7.0 25
1 M C -0.37 7.0 25
1 M H -1.73 7.0 25
1 M R -0.70 7.0 25
1 M K -1.28 7.0 25
1 M Q -1.45 7.0 25
1 M E -0.59 7.0 25
1 M N -1.26 7.0 25
1 M D 0.20 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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