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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
260 L V -0.58 7.0 25
260 L I -0.88 7.0 25
260 L M -1.25 7.0 25
260 L F -0.41 7.0 25
260 L W -0.18 7.0 25
260 L Y -0.62 7.0 25
260 L G -4.69 7.0 25
260 L A -3.34 7.0 25
260 L P -1.24 7.0 25
260 L S -3.56 7.0 25
260 L T -3.40 7.0 25
260 L C -3.64 7.0 25
260 L H -1.23 7.0 25
260 L R -2.63 7.0 25
260 L K -2.74 7.0 25
260 L Q -2.00 7.0 25
260 L E -1.16 7.0 25
260 L N -2.91 7.0 25
260 L D -3.06 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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